Abstract: Objective To establish a transgenic Fah- / -liver injury mouse model and explore the mechanism of liver injury. Method SPF C57BL / 6J wild-type mice were selected as WT group. According to the result of gene identification, 24 Fah- / -homozygous mice aged 6-8 weeks were selected and divided into the NTBC drinking water group, the NTBC drinking water stop group for one week, and the NTBC drinking water stop group for two weeks. The weight was weighed, blood was collected, serum was isolated, liver function biochemical indexes were tested, and the animals were sacrificed. The expression of Fah protease and Fah protein were detected by immunohistochemistry and Western blot. Result After gene identification, 24 Fah - / - homozygous mice were successfully bred. The result of subsequent study on the mechanism of liver injury showed that ALT, AST and TBIL of liver function in the one-week and two-week NTBC cessation groups were significantly increased compared with those in the normal NTBC group, while ALB was significantly decreased, with statistical significance. Pathological HE staining result of liver tissue showed that the structure of liver tissue in WT group and normal NTBC group was normal, while different degrees of lesions such as hepatocyte hypertrophy, necrosis and inflammatory cell infiltration were observed in NTBC group. The longer the time of NTBC cessation, the more serious the degree of liver injury. The result of liver immunohistochemistry showed that the expression of Fah protease in WT group was strongly positive, the expression of Fah protease in normal NTBC group was negative, and the expression of Fah protease in hepatocytes of the one-week and two-week NTBC stop groups was weakly positive. The expression of Fah protein by Western blot was consistent with that by immunohistochemistry. Conclusion The Fah- / - mouse model was successfully established and the mechanism of liver injury was studied, so as to provide expe rimental data reference forsubsequent studies.

Key words: Fah - / - mice, NTBC, liver injury, Fumarate acetoacetic acid hydrolase ( Fah)

摘要： 目的 建立转基因 Fah - / -肝损伤小鼠模型,探讨其肝损伤的机制。 方法 选取 SPF 级 C57BL / 6J 野生型小鼠作为 WT 组。 取转基因 Fah+ / -肝损伤杂合子小鼠培育后,根据基因鉴定结果选取 6 ~ 8 周龄的 Fah - / - 纯合子小鼠 24只,分为正常给予 NTBC 饮水组、停 NTBC 饮水 1 周组和停 NTBC 饮水 2 周组,称量体质量,采血分离血清,进行肝功能生化指标检测,处死动物,取肝组织进行病理学检查、免疫组化检测 Fah 蛋白酶的表达及采用 Western blot 方法检测 Fah 蛋白表达。 结果 经基因鉴定,成功繁育 Fah - / -纯合子小鼠 24 只,后续用于肝损伤机制研究结果表明,停 NTBC 1 周组、停 NTBC 2 周组肝功能生化指标 ALT、AST、TBIL 与正常给予 NTBC 组比较显著升高,而 ALB 显著降低,差异有统计学意义。 肝组织病理 HE 染色结果表明,WT 组及正常给予 NTBC 组肝组织结构正常,而停 NTBC组出现肝细胞肥大、坏死及炎性细胞浸润等不同程度的病变,停 NTBC 时间越长,肝损伤的程度越严重;肝组织免疫组化结果表明,WT 组 Fah 蛋白酶表达为强阳性,正常给予 NTBC 组 Fah 蛋白酶表达阴性,停 NTBC 1 周组和停NTBC 2 周组肝细胞 Fah 蛋白酶表达弱阳性。 Fah 蛋白表达结果与免疫组化结果基本符合。 结论 成功建立 Fah- / -小鼠模型并对其肝损伤机制研究,为后续研究提供实验数据参考。

关键词: Fah - / -小鼠, NTBC, 肝损伤, 延胡索酸乙酰乙酸水解酶( Fah)